聯系電話:512-67626747 聯系郵箱:eegene215@qq.com
                            翊準國際生物科技/
                            動態分子監測 產品系列

                            SIGNIFICANT OVEREXPRESSION OF DVL1 IN TAIWANESE COLORECTAL C

                            發布時間:2016-10-24 ??? 閱讀數:

                             Significant Overexpression of DVL1 in Taiwanese Colorectal Cancer Patients with Liver Metastasis

                             
                            Ming-Yii Huang 1,2,3, Li-Chen Yen 4, Hsueh-Chiao Liu 5, Po-Ping Liu 6, Fu-Yen Chung 4,
                            Tsu-Nai Wang 7, Jaw-Yuan Wang 3,8,9,10,* and Shiu-Ru Lin 4,11,*
                             
                            Received: 1 August 2013; in revised form: 24 September 2013 / Accepted: 29 September 2013 /
                            Published: 14 October 2013
                             
                            Abstract: Undetected micrometastasis plays a key role in the metastasis of cancer in colorectal cancer (CRC) patients. The aim of this study is to identify a biomarker of CRC patients with liver metastasis through the detection of circulating tumor cells (CTCs). Microarray and bioinformatics analysis of 10 CRC cancer tissue specimens compared with normal adjacent tissues revealed that 31 genes were up-regulated (gene expression ratio of cancer tissue to paired normal tissue > 2) in the cancer patients. We used a weighted enzymatic chip array (WEnCA) including 31 prognosis-related genes to investigate CTCs in 214 postoperative stage I–III CRC patients and to analyze the correlation between gene expression and clinico-pathological parameters. We employed the immunohistochemistry (IHC) method with polyclonal mouse antibody against DVL1 to detect DVL1 expression in 60 CRC patients. CRC liver metastasis occurred in 19.16% (41/214) of the patients. Using univariate analysis and multivariate proportional hazards regression analysis, we found that DVL1 mRNA overexpression had a significant, independent predictive value for liver metastasis in CRC patients (OR: 5.764; 95% CI: 2.588–12.837; p < 0.0001 on univariate analysis; OR: 3.768; 95% CI: 1.469–9.665; p = 0.006 on multivariate analysis). IHC staining of the immunoreactivity of DVL1 showed that DVL1 was localized in the cytoplasm of
                            CRC cells. High expression of DVL1 was observed in 55% (33/60) of CRC tumor specimens and was associated significantly with tumor depth, perineural invasion and liver metastasis status (all p < 0.05). Our experimental results demonstrated that DVL1 is significantly overexpressed in CRC patients with liver metastasis, leading us to conclude that DVL1 could be a potential prognostic and predictive marker for CRC patients.
                             
                            Keywords: colorectal cancer; liver metastasis; DVL1 overexpression; weighted enzymatic chip array (WEnCA); immunohistochemistry (IHC)

                            上一篇:DPYD, TYMS, TYMP, TK1, AND TK2 GENETIC EXPRESSIONS AS RESPON
                            下一篇:DPYD, TYMS, TYMP, TK1, AND TK2

                            • 蘇州翊準基因科技
                              中國江蘇省蘇州市工業園區新平街388號B幢B-203(騰飛創新園塔樓B-203)
                              電話:86-512-67626747
                              傳真:86-512-67626747

                            Copyright © 2016 蘇州翊準基因科技有限公司 版權所有 蘇ICP備17013352號

                            国产超碰人人爽人人做人人添,精油按摩,免费黄色,人妻精品动漫h无码中字 <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <蜘蛛词>| <文本链> <文本链> <文本链> <文本链> <文本链> <文本链>